B4930
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 | Sequencing reaction was purified using a SigmaSpin 96-well plate. pFLAG™ MAC plasmid (Sigma, E 5644) was sequenced using BigDye™ Terminator v 3.0 chemistry (20 μl total reaction volume; BigDye premix diluted 1:1 with SeqSaver™ [Sigma, S 3938]). Data was generated on an ABI Prism® 3700 DNA Analyzer with POP-6™ polymer and 10X CE Buffer (Sigma, B 4930). Phred 20 > 600. |
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B4930
Sigma
Capillary Electrophoresis Running Buffer (10x)
for automated DNA sequencing
Description
| Features and Benefits | • Cost efficient - high quality buffer at a great value • Quality control testing of every lot ensures proper pH, conductivity and performance for Capillary Electrophoresis Sequencing • Compatible with ABI Prism® 3700, 3100 and 310 DNA sequencers • Room temperature stability for convenient shipping and storage • Plastic bottles provide easy handling • 1, 4 and 20 L as well as custom packaging options available |
| General description | Sigma’s 10× Capillary Electrophoresis Running Buffer was designed to meet the high quality and high throughput demands of today’s sequencing labs. As a primary manufacturer and leading supplier of TBE to the gel-based sequencing community Sigma has the know how and large scale capacity to meet your buffer needs. Scientists at Sigma have taken this experience and joined it with today’s advancing technologies to optimize a buffer for use with capillary electrophoresis sequencing instruments. |
Properties
| type | for automated DNA sequencing |
Safety
| WGK Germany | 3 |
